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詳細解析Biolegend流式細胞的工作原理
發(fā)布時間： 2022-02-21 點擊次數(shù)： 1083次Biolegend流式細胞技術(shù)是利用流式細胞儀進行的一種單細胞定量分析和分選技術(shù)。流式細胞術(shù)是單克隆抗體及免疫細胞化學技術(shù)、激光和電子計算機科學等高度發(fā)展及綜合利用的高技術(shù)產(chǎn)物。它是在細胞分子水平上通過單克隆抗體對單個細胞或其他生物粒子進行多參數(shù)、快速的定量分析。它可以高速分析上萬個細胞，并能同時從一個細胞中測得多個參數(shù)，具有速度快、精度高、準確性好的優(yōu)點，是當代*的細胞定量分析技術(shù)之一。基本結(jié)構(gòu)：Biolegend流式細胞主要是由三部分構(gòu)成：1、液流系統(tǒng)，包括流動室和液流驅(qū)動系統(tǒng)；2、光學系統(tǒng)，包括激發(fā)光源和光束收集系統(tǒng)；3、電子系統(tǒng)，包括光電轉(zhuǎn)換器和數(shù)據(jù)處理系統(tǒng)。Biolegend流式細胞儀的工作原理：是使懸浮在液體中分散的經(jīng)熒光標記的細胞或微粒逐個通過樣品池，同時由熒光探測器捕獲熒光信號并轉(zhuǎn)換成分別代表前向散射角、側(cè)向散射角和不同熒光強度的電脈沖信號，經(jīng)計算機處理形成相應(yīng)的點圖，直方圖和加三維結(jié)構(gòu)圖像進行分析。用于FCM的樣本是單細胞懸液，可以是血液、懸浮細胞培養(yǎng)液、各種體液、新鮮實體瘤的單細胞懸液以及石蠟包埋組織的單細胞懸液等。
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